Contact an Account ManagerDetermination of arterial-venous differences and leg efflux of 3-methylhistidine using ion pair HPLC and postcolumn fluorescence derivatization
Summary
Type: HPLC
Application #: 107550
Catalog#: 720018.46 - MN HPLC Column, Nucleosil C18, 100A, 7um, 4.6x250mm
Detection: postcolumn derivatization with OPA reagent, flow rate 1ml/min; detection: fluorescence, excitation 350nm, emission 420nm
Conditions: eluent: 1.0l 5mM Na heptanesulphonate, plus 4.0ml conc. acetic acid, adjusted to pH 4.0 with conc. NaOH, filtered through 0.45μm membrane filter, methanol added to 4%
flow rate 0.8ml/min
Authors: Andersson E
Source: J Chromatogr 414 (1987) 174 - 179
Details
Legend: Fig. 1. Chromatograms of A) unspiked plasma sample and B) standard solution of
3-methylhistidine (*).